ABSTRACT
Objective To analyze the epidemiological characteristics of mumps in Zaozhuang city from 2010 to 2017, and establish a predictive model to predict prevalence trend, so as to provide scientific basis for the prevention and control of mumps. Methods Mumps cases between 2010 and 2017 screened from Infectious Diseases Report Information Management System of Chinese Information System of Disease Prevention and Control were analyzed by the descriptive epidemiologic method. The time series analysis method was applied to model and predict the trend of disease. Results A total of 6 353 mumps cases were reported in Zaozhuang city from 2010 to 2017. The average annual incidence was 19.75/100 000. The incidence of men was higher than that of women. The seasonal indices for December to next January and April to July were all above 1. The incidence rates of Shizhong and Yicheng were higher than in other areas. The incidences aged from 3 to 14 accounted for 76.85 % of the total number of cases, while students, scattered children and childcare accounted for 86.11%. The established time series model was ARIMA (1, 0, 0) * (0, 0, 0)12. The mean absolute percentage error of the predictive values based on the model was 39.99% and the number of cases predicted by the model in 2018 was 471. The predictive incidence rate was 11.13/100 000. Conclusions Primary and secondary school students and scattered children are the main population for the prevention and control of mumps. The model obtains better forecasting results and can be used for short-term prediction and dynamic analysis of mumps.
ABSTRACT
Objective: To investigate the effects of histone deacetylase inhibitor trichostatin A (TSA) on radiosensitivity of human cervical cancer cell line HeLa under hypoxia condition. Methods: Under hypoxia condition, the HeLa cells were treated with different concentrations of TSA for 12, 24, 48 and 72 h, respectively. The survival rate of HeLa cells was detected by MTT assay. The half inhibitory concentration (IC50) and IC10 values for TSA were calculated. The radiosensitivity in HeLa cells treated with TSA (IC10) for 24 h under hypoxia condition was detected by colony formation assay. The expressions of hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) proteins were measured by immunocytochemistry. Results: The survival rate of HeLa cells induced by TSA and hypoxia was decreased in a concentration and time-dependent manner. The radiosensitivity of HeLa cells under hypoxia condition was increased by treatment with TSA (IC10) for 24 h (P <0.05). The expressions of HIF-1α and VEGF proteins were higher in HeLa cells exposed to hypoxia than that in HeLa cells exposed to normoxia. The expressions of HIF-1α and VEGF proteins in HeLa cells under hypoxia condition were decreased by treatment with TSA (IC10) for 24 h. Conclusion: TSA can significantly enhance the radiosentivity of HeLa cells under hypoxia condition. This effect may be related with the down-regulation of HIF-1α and VEGF protein expression. Copyright© 2011 by the Editorial Board of Tumor.